GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
应用类型
ELISA (ELISA), Dot Blot, Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)
免疫原
KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria P42212.
来源宿主
小鼠
反应性
Native and denatured forms of GFP and Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP)
