中文名称

TAMRA phosphoramidite, 5-isomer

英文名字

TAMRA phosphoramidite, 5-isomer

供应商

antibodies

产品货号

ANT-A270325-250

产品报价

￥询价/250mg

产品说明书

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购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系。本公司所有产品仅供科研使用，不用于临床诊断。

产品新闻

背景资料

该TAMRA phosphoramidite, 5-isomer的背景资料请参考antibodies.com的说明书

产品描述

This phosphoramidite is used for synthesis of oligonucleotides 5'-labeled with TAMRA. TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5'-terminal TAMRA and FAM in the middle of the sequence or at the 3'-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively). TAMRA 5'-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently. The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection. Coupling takes only 7.5 minutes. Deprotection should be performed with tret-buthylamine : methanol : water at a ratio of 1 : 1 : 3 (v/v/v) ("TAMRA cocktail") for 6 hours at 60 °C, then cooled down to room temperature. Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.

产品形式

保存建议

Shipped at room temperature. Upon delivery, store in the dark at -20

其他

antibodies.com 以具有成本效益的价格向生命科学研究人员提供高质量的生物试剂。公司总部位于剑桥（英国），并在斯德哥尔摩（瑞典）和圣路易斯（美国密苏里州）设有办事处，可提供包括一抗、二抗、蛋白质和肽、免疫分析和试剂盒以及其他相关产品。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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