Synaptic vesicle membrane docking and fusion is mediated by SNAREs （soluble N-ethylmaleimide-sensitive factor attachment protein receptors） located on the vesicle membrane （v-SNAREs） and the target membrane （t-SNAREs）. The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices， one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane， the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore， SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein （SNAP-25） is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus， SNAP-25 is directly involved in the release of neurotransmitters.
应用类型
ELISA， Western blot
免疫原
Anti-human SNAP25 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SNAP25 amino acids 1-206 purified from E. coli.
