UCHL1 （PGP9.5） belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. Protein Gene Product （PGP9.5） is a neuron specific protein， structurally and immunologically distinct from neuron specific enolase. Human UCHL1 and UCHL3 have an extremely complicated knot structure for a protein， with five knot crossings. It is considered that the knot structure may increase a protein’’s resistance to degradation in the proteasome. The protein， which has a molecular weight of 27 kDa was first defined by high resolution two dimetional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system， in many neurodcrine cells， in segments of the renal tubules， in spermatogonia and leydig cells of the testis， in ova and in some cells of both the pregnant and non pregnant corpus luteum. A point mutation （I93M） in UCHL1 is implicat
应用类型
ELISA， Western blot and immunohistochemistry
免疫原
Anti-human PGP9.5 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PGP9.5 amino acids 1-223 purified from E. coli.
