Human Pin 1 is a peptidyl-prolyl cis/trans isomerase （PPIase） that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain， and is structurally and functionally related to Ess1/Ptf1， an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators （Cdc25 phosphatase and NIMA， PLK I， Wee， and Myt1 kinases）， several transcription factors like B-Catenin， c-Jun， and the tumor suppressor protein p53， and some specific proteins like the RNA Pol II， the cytoskeleton protein tau， and the G1/S protein Cyclin D1.
应用类型
ELISA， Western blot
免疫原
Anti-human PIN1 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PIN1 amino acids 1-163 purified from E. coli.
