Geminin is a 25 kDa nuclear protein， which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1， and is involved in neural differentiation. In addition， Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the “S phase” of the cell cycle. Inhibition of Geminin during the “S phase” （by RNAi） results in an additional round of replication of portions of the genome. During the “M phase” of the cell cycle （mitosis） Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover， inhibition of Geminin during mitosis （by RNAi） causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. G
应用类型
ELISA， Western blot
免疫原
Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli.
