Aurora Kinase B （AURKB） belongs to a family of mitotic serine/threonine kinases. AURKB connects with chromosomes for the period of prophase prior to relocalizing to the spindle at anaphase. AURKB localizes to microtubules near kinetochores， specifically to the specialized microtubules called K-fibers. AURKB controls chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. AURKB is required for kinetochore localization of BUB1 and SGOL1. AURKB expression during the G2/M phase transition is firmly coordinated with histone H3 phosphorylation， while overexpression is seen in many kinds of cancers. AURKB phosphorylates ’’Ser-10’’ and ’’Ser-28’’ of histone H3 during mitosis. AURKB is a component of the CPC （chromosomal passenger complex）， which is a complex that acts as a key regulator of mitosis. High level expression of AURKB is seen in the thymus， which is also expressed in the spleen， lung， testis， colon， placenta and fetal liver. AURKB is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
应用类型
ELISA and Immunofluorescence
免疫原
Anti-human AURKB mAb， is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human AURKB amino acids 1-344 purified from E. coli.
