EGLN1 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor （HIF） alpha proteins. HIF is a transcriptional complex which has a vital role in mammalian oxygen homeostasis. PHD1 （Egln2）， PHD-2 （Egln1）， and PHD3 （Egln3） belong to the Egln family of proline hydroxylases. The EGLN family that is known to be a cellular oxygen sensor initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 hydroxylate both proline 402 and proline 564， however PHD3 can only hydroxylate proline 564. Other than HIF， PHD enzymes catalyze the hydroxylation of RNA polymerase subunits and myogenin. The EGLN1 protein functions as a cellular oxygen sensor. Under normal oxygen concentration， modification by prolyl hydroxylation is a key regulatory event which targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in the EGLN1 gene are linked to erythrocytosis familial type 3 （ECYT3）.
应用类型
ELISA， Western blot
免疫原
Anti-human EGLN1 mAb， is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human EGLN1 amino acids 271-426 purified from E. coli.
