Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL35 (mitochondrial ribosomal protein L35)encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to MRPL35 are found on chromosomes 6p, 10q, and Xp.蛋白别名为:MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35;基因ID为:51318;蛋白质ID:Q9NZE8
应用类型
WB,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.
免疫原
合成多肽:the Internal region of human MRP-L35. at AA rangle: 80-160