This intronless gene(CEBPA) encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein(CCAAT/enhancer binding protein alpha) functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of CEBPA is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.蛋白别名为:CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha;基因ID为:1050;蛋白质ID:P49715
应用类型
WB,IHC-p,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:5000. Not yet tested in other applications.
免疫原
合成多肽:human C/EBP α around the non-phosphorylation site of T226.