- 中文名称
Antioxidant Assay Kit, BioAssay
- 英文名字
- Antioxidant Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- A2298-44G
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- An Antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer's disease, Parkinson's disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline.Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery. The improved assay measures total antioxidant capacity in which Cu2+ is reduced by antioxidant to Cu+. The resulting Cu+ specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample.Key Features:Sensitive and accurate. Use 20ul sample. Linear detection range from 1.5 to 1000uM Trolox equivalents.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Applications:Direct Assays: serum, plasma, urine, saliva and other biological samples, food and beverages.Drug Discovery/Pharmacology: effects of drugs on TAC.Kit Contents:Reagent A: 12ml Reagent B: 1mlStandard: 100ul 50mM TroloxStorage conditions. The kit is shipped at room temperature. Store Reagent A at room temperature and other components at -20 °C. Shelf life of six months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Sample Preparation:Samples should not contain any metal chelators (e.g. EDTA) and should be clear and free of any turbidity or particles. Liquid samples (e.g. nonhemolyzed serum, plasma) can be assayed directly. Cell lysate is prepared by homogenizing or sonicating cells in ice-cold 1 x PBS and centrifugation for 10 min at 14,000 rpm to pellet any debris. Use the clear supernatant for the assay. If not assayed immediately, freeze supernatant at -80°C (stable for 1 month).Assay Procedure:1. Standards and Samples. Equilibrate all components to room temperature. Briefly centrifuge Reagent B and Standard before opening. Mix 5ul of the standard with 245ul dH2O (final 1mM Trolox). Dilute standards as shown in the Table below. Transfer 20ul standards into wells of a clear flat-bottom 96-well plate.No 1mM Trolox+H2O Vol (ul) Trolox (uM)1 100ul+0ul 100 10002 60ul+40ul 100 6003 30ul+70ul 100 3004 0ul+100ul 100 0Transfer 20ul of each sample into separate wells of the 96-well plate.Note: for unknown samples, perform several dilutions to ensure that TAC is within the linear range of 1.5 to 1000uM Trolox equivalents.2. Assay. Prepare enough Working Reagent for Sample and Standard wells by mixing, for each assay well, 100ul Reagent A and 8ul Reagent B. Add 100ul Working Reagent to all assay wells. Tap plate to mix. Incubate 10 min at room temperature.3. Read OD570nm on a plate reader.Note: if calculated TAC is higher than 1000uM Trolox equivalents, dilute sample in dH2O and repeat assay. Multiply the results by the dilution factor.Calculation:Subtract blank OD value (#4) from all standard and sample OD values. Plot the DOD570nm against standard concentrations and determine the slope of the standard curve. Calculate the Total Antioxidant Capacity (TAC) of Sample,TAC =ODSAMPLE-ODBLANKSlope (uM-1)x n (uM Trolox Equivalents)ODSAMPLE and ODBLANK are the OD570nm values of the sample and H2O blank (standard #4). n is the sample dilution factor.Materials Required, But Not Provided:Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, plate reader capable of reading optical density at 570nm, homogenizer or sonicator etc.
- 产品特点
- 针对Antioxidant Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Antioxidant Assay Kit, BioAssay产品后将其置于RT/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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