Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).|Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.|IL-6 is a protein of 212 amino acids. It’s a pleiotropic cytokine which has an impact on the regulation of immune and nonimmune events. It is produced by the monocytes and macrophages in response to other infl ammatory cytokines like Interleukin-11 (IL-11) and the Tumor Necrosis Factor-beta (TNF-β).|IL-6 acts on immune and nonimmune cells: it helps to differentiate T-cells from B-cells but it is also vital for the development of blood cells (white cells, red cells or platelets).|Due to these target cells, IL-6 is an activator of immune system and infl ammation. It also controls the metabolism by action on adipocytes, the bone metabolism and the pain.|A dysregulation in IL-6 production induces many disorders such as autoimmune diseases, infl ammation diabetes or cancer.
