中文名称

【ZAP70】ZAP70蛋白，有活性的（5 ug）

英文名字

ZAP70, Active

供应商

Biovision

产品货号

7752-5

产品报价

￥已停产

产品说明书

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产品新闻

背景资料

ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. ZAP70 tyrosine kinase is tyrosine phosphorylated in Jurkat T cells and in purified peripheral T cells after MHC-I ligation. The phosphorylation of ZAP70 after MHC-I ligation is dependent on TCR/CD3 surface expression. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2). Another substrate of ZAP70 is LAT (linker for activation of T cells). Direct tyrosine phosphorylation of LAT with activated protein-tyrosine kinase Zap70 is necessary and sufficient for the association and activation of signalling proteins. Zap-70 efficiently phosphorylates LAT on tyrosine residues at positions 226, 191, 171, 132 and 127. By substituting these tyrosine residues in LAT with phenylalanine and by utilizing phosphorylated peptides derived from these sites, the tyrosine residues in LAT have been shown to be required for the direct interaction and activation of Vav, p85/p110α and phospholipase Cγ1 (PLCγ1) (3).

序列

ZAP70蛋白,有活性的蛋白产品，又称ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, Syk-related tyrosine kinase.其对应NCBI基因号为6788，NCBI基因别称为STK3源，对应Accession号为Q13188.该蛋白产品又称ZAP 70, ZAP-70, Active ZAP70, ZAP70, ZAP, human ZAP70, active kinase, active kinases, recombinant ZAP70, recombinant ZAP, kinase activity assay, protein kinase等.

来源宿主

提供的【ZAP70】ZAP70蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.

溶解建议

提供的ZAP70蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.

保存建议

在您收到艾美捷发出的【ZAP70】ZAP70蛋白,有活性的产品后，建议将其置于-80°C保存.

其他

BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的Leader，BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供优质、新的细胞生物学研究工具而不断扩大产品及服务质量.

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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