Major histocompatibility complex （MHC） class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides （CLIP） from an antigen binding groove in MHC II å/∫ dimers through the activity of MHC molecules HLA-DM and -DO，and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells，HLA-DM，-DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules （HLA-DR，-DQ and -DP） by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis （RA） patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain （∫-2-Microglobulin）. Polymorphisms yield hundreds of HLA-B and -C alleles.
应用类型
WB: 1:500 – 1:16000.
免疫原
This HLA-DRA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from human HLA-DRA.
