The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
序列
重组人PTP1B 蛋白产品,又称Protein tyrosine phosphatase, non-receptor type 1, PTPN1, Protein-tyrosine phosphatase 1B, Human Recombinant PTP1B,.其对应NCBI基因号为5770,对应Accession号为P18031.
来源宿主
提供的【PTP1B 】重组人PTP1B 产品的来源为大肠杆菌重组表达.
溶解建议
重组人PTP1B 产品为液体即用型,其溶液组成为:1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β mercaptoethanol, 1 mM EDTA, 20% Glycerol.