Peroxiredoxin （Prx） is a growing peroxidase family，whose mammalian members have been known to connect with cell proliferation，differentiation，and apoptosis. Many isoforms （about 50 proteins），collected in accordance to the amino acid sequence homology，particularly amino-terminal region containing active site cysteine residue，and the thiol-specific antioxidant activity，distribute throughout all the kingdoms. Among them，mammalian Prx consists of 6 different members grouped into typical 2-Cys，atypical 2-Cys Prx，and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup，the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase （TPx） activity utilizing thioredoxin，thioredoxin reductase，and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I，II，and VI in cytosol，Prx III in mitochondria，Prx IV in ER and secretion，Prx V showing complicated distribution including peroxisome，mitochondria and cytosol. Prx V is involved in reduction of hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
应用类型
Western blot: 1:1000， IP: 1-2 ul， ICC: 20 ul/ml， ELISA.
免疫原
Recombinant human Prx V fragment protein （without mitochondrial leader sequence） purified from E.coli.
