The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.
应用类型
ELISA,
免疫原
Anti-Ad-9 E4 Orf1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide near the C-terminus of the Ad-9 E4 Orf1.
来源宿主
Rabbit
反应性
Virus
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
