Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of the histone H2A variant H2A.Z is associated with the promoters of active genes. Anti-H2A.Zac Antibody is ideal for research in Chromatin Remodeling and Epigenetics.
应用类型
ELISA,Chip,IF Microscopy,Western Blot,
免疫原
Anti-H2A.Zac Antibody was produced in rabbits by repeated immunization with a synthetic peptide containing the region of human histone H2A.Z acetylated at lysines 5, 7 and 11.
来源宿主
Rabbit
反应性
H. sapiens (Human); Mus musculus (Mouse); Wide range expected
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
