SMO (Smoothened, Frizzled Class Receptor) receptors are non-classical G protein-coupled receptors that probably associates with the patched protein (PTCH) to transduce the hedgehogs proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). It is required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. And it interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation. Diseases associated with SMO include Curry-Jones Syndrome and Basal Cell Carcinoma. Anti-Smoothened Antibody is useful for researchers interested in Cancer research.
应用类型
ELISA,Immunohistochemistry,Western Blot,
免疫原
Anti-Smoothened antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Smoothened conjugated to Keyhole Limpet Hemocyanin (KLH).
来源宿主
Rabbit
反应性
H. sapiens (Human)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
