Inositol-requiring enzyme 1 (IRE1) or ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1) is a Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain, and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Diseases associated with ERN1 include Chromosome 6Pter-P24 Deletion Syndrome and Alzheimer Disease. Anti-IRE1 Antibody is useful for researchers interested in Neuroscience research.
Anti-Ire1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Ire1 conjugated to Keyhole Limpet Hemocyanin (KLH).
来源宿主
Rabbit
反应性
H. sapiens (Human); Mus musculus (Mouse)
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
