ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.
应用类型
ELISA,Flow Cytometry,Western Blot,
免疫原
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.
来源宿主
Rabbit
反应性
H. sapiens (Human)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
