Adipocytes are highly active cells with potent autocrine,paracrine,and endocrine functions. Adiponectin is a serum protein produced and secreted exclusively by adipose tissues. Unlike insulin and leptin,adiponectin levels in plasma remain constant throughout the day and are not acutely affected by food intake.
应用类型
Western blotting (0.5-2 ug/ml) and Immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually. Recombinant human adiponectin (Cat.# 4901-25) can be used as a positive control. The antibody detects the monomer (30 kDa), dimmer (60 kDa), and multimers (90 kDa) of adiponectins in samples from human, mouse, and rat origins.
免疫原
E. coli expressed recombinant human adiponectin
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Adiponectin蛋白发生免疫反应.
保存建议
提供的Adiponectin抗体产品为250 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Adiponectin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.