Interleukin-12 is a disulfide-linked heterodimeric protein (p70),composed of two subunits,p35 and p40,which are encoded by two different genes. Recombinant Human IL-12 p40 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 306 amino acids fragment (23-328).
应用类型
Western blot analysis (0.5-4 ug/ml). It detects mainly the 40 kDa of IL-12p40 isoform, and in a lesser extent the 35 kDa of IL-12p35 isoform. Recombinant human IL-12 (Cat.#:4732-10) can be used as a positive control. However, the optimal conditions should be determined individually.
免疫原
E. coli expressed recombinant human IL-12
来源宿主
兔
反应性
经检测验证,可与人种属来源的IL-12p40蛋白发生免疫反应.
保存建议
提供的IL-12p40抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该IL-12p40抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.