MIF （macrophage migration inhibitory factor） was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of microphages. Recently，MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF also plays a critical role in the host control of inflammation and immunity.
应用类型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （5-10 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 67 of human MIF
来源宿主
兔
反应性
经检测验证，可与小鼠、大鼠种属来源的MIF蛋白发生免疫反应.
保存建议
提供的MIF抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该MIF抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
