Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity，metabolism and body temperature. cDNA clones encoding leptin have been isolated from human，simian，mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acide residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction，immunological response and neuroendocrine signaling.
应用类型
Western blot analysis （0.5-2 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been tested.
免疫原
E. coli-expressed recombinant mouse Leptin
来源宿主
兔
反应性
经检测验证，可与小鼠种属来源的Leptin蛋白发生免疫反应.
保存建议
提供的Leptin抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Leptin抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
