Rat MCP-1/JE （monocyte chemoattractant protein-1） is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. The 3’-end is a serine and threonine rich zone，which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight （25 kDa）. In vitro，MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils，but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
应用类型
Western blot analysis （0.5-2 ug/ml）. From researcher’s feedback， it can also be used for neutralization studies. However， the optimal conditions should be determined individually.
