Calmodulin binding peptide (CBP) tag is a fusion protein that is useful for immunoaffinity purification of proteins expressed in E.coli. The CBP tag binds to calmodulin resin in the presence of low concentration of calcium and can be eluted with a neutral buffer. The antibody is suitable for identifying CBP-fusion proteins.
应用类型
The antibody can be used for Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide (Cat.# 3960BP-50) is available separately.
免疫原
A synthetic CBP tag peptide KRRWKKNFIAVSAA conjugated to KLH.
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的CBP标签蛋白发生免疫反应
保存建议
提供的100 ug CBP-Tag抗体产品为无色溶液其浓度为0.5mg/ml溶解于包含有30%甘油, 0.5% BSA, 5 mM EDTA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该CBP-Tag抗体产品置于-20 °C冷冻保存,建议分装使用以避免反复冻融.