Methylation of DNA at cytosine residues plays an important role in regulation of gene expression，genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date，3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1，Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality.
应用类型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Rat kidney tissue lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 1208 of rat DNMT1.
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的DNMT1蛋白发生免疫反应.
保存建议
提供的DNMT1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该DNMT1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
