Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. ATG16，another member of the autophagy protein family，forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain，it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined.
免疫原
Synthetic peptide surrounding amino acid 565 of human APG16
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的APG16/ATG16蛋白发生免疫反应.
保存建议
提供的APG16/ATG16抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞的PBS缓冲液中.请将该APG16/ATG16抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
