Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. ATG12，another member of the autophagy protein family，forms a conjugate with ATG5； this conjugate has a ubiquitin-protein ligase （E3）-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA （also known as IPS-1），inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 21 of mouse APG12
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的APG12/ATG12蛋白发生免疫反应.
保存建议
提供的APG12/ATG12抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞的PBS缓冲液中.请将该APG12/ATG12抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
