Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is APG10 （Also called ATG10，APG10L），an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3，a homolog of yeast Apg8，to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined.
免疫原
Synthetic peptide， near the carboxyl terminus of human APG10
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的APG10/ATG10蛋白发生免疫反应.
保存建议
提供的APG10/ATG10抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞nn的PBS缓冲液中.请将该APG10/ATG10抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
