FoxH1 (also known as Forkhead box H1 or FAST-1) is a transcription factor that shares close homology with Xenopus FAST-1. FoxH1 binds to SMAD2/4 and activates activin response through a DNA binding motif region that consist of TGT(G/T)(T/G)ATT sequence. FoxH1 binding to Smad2/Smad4/FAST1 forms a transcriptionally complex structure that can induced TGFB signal transduction.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~44kDa of FoxH1 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 55 of mouse FoxH1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的FoxH1蛋白发生免疫反应.
保存建议
提供的FoxH1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该FoxH1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.