Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 226 of rat Apg5
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、猪、牛种属来源的Apg5/Atg5蛋白发生免疫反应.
保存建议
提供的Apg5/Atg5抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Apg5/Atg5抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
