SAPK/Erk kinase （SEK1），also known as MKK4 or Jun kinase kinase （JNKK），activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261，respectively，by MEKK. Like MEK，SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human， mouse， and dog origins.
免疫原
Synthetic peptide surrounding amino acid 347 of mouse SEK1
来源宿主
兔
反应性
经检测验证，可与人、小鼠、狗种属来源的SEK1/MKK4/JKK1蛋白发生免疫反应.
保存建议
提供的SEK1/MKK4/JKK1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该SEK1/MKK4/JKK1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
