The Firefly luciferase is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis endemic to Yunnan province,China. It has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis as well as a broader and more physiologically relevant effective pH range. Luciferase has been used as a sensitive reporter for studying gene regulation and function. Firefly luciferase catalyzes the oxidative carboxylation of luciferin,a reaction with the highest efficiency of any known bioluminescence reaction.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes Chinese firefly luciferase. Cross-reactivity to other types of Luciferases have not been determined. Recombinant Chinese firefly luciferase (Cat.# 7901-5) can be used as a positive control.
免疫原
Recombinant Chinese Firefly luciferase
来源宿主
兔
反应性
经检测验证,可与Luciferase种属来源的Luciferase蛋白发生免疫反应.
保存建议
提供的Luciferase抗体产品为200 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Luciferase抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.