Lactate dehydrogenase (LDH) contains two isoforms in mammals,LDH-M and LDH-H,which come together to form a homotetramer of 36 kDa subunits. LDH is often as a marker for tissue breakdown or cytotoxicity. LDH shows cytoplasm localization.
应用类型
Western blotting (1:500-2500). However, the optimal conditions should be determined individually. The antibody recognizes ~36 kDa LDH. HeLa cell lysate can be used as a positive control.
免疫原
Full length LDH isolated from rabbit muscle
来源宿主
山羊
反应性
经检测验证,可与人、小鼠、大鼠种属来源的LDH蛋白发生免疫反应.
保存建议
提供的LDH抗体产品为500 ug 无色溶液,其浓度为5.0mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该LDH抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.