Fibroblast growth factor receptor substrate 2 （FRS2） is a 70-90kDA member of the FSR family of lipid-anchored docking protein. FRS2 contains Grb2 binding sites，a myristylation sequence and a PTP domain. Activation of FGFR leads to tyrosine phosphorylation of FRS2 and the binding of phosphorylated FRS2 to GRB2/SOS complexes. Once phosphorylated，FRS2 recruits SH2 domain-containing proteins including Grb2 and SHP-2 mediating downstream signalling. FRS2 thus，acts as an intermediary between FGF and Trk receptors that links receptor tyrosine kinase to Ras/MAPK signaling pathway.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 493 of human FRS2
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的FRS2蛋白发生免疫反应.
保存建议
提供的FRS2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该FRS2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
