Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3’-phosphate-5’-phosphosulfate. Sulfurylated steroids do not bind to the estrogen receptor with high affinity and，therefore，are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor-active estrogens.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined. Detects 31 kDa Estrogen Sulfotransferase （EST） of human and rat origins.
免疫原
Synthetic peptide surrounding amino acid 195 of human Estrogen Sulfotransferase （EST）
