Sample Type：Serum，Plasma，Biological FluidsIntended Use：This BioAssay kit is a competitive ELISA for in vitro quantitative measurement of Ang1-7 in human serum，plasma and other biological fluidsSensitivity：9.375pg/mLRange：15.625-1000pg/mLSpecificity：HumanTest Principle：This BioAssay ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with human Ang1-7 （Angiotensin 1-7）. During the reaction，human Ang1-7 （Angiotensin 1-7） in the sample or standard competes with a fixed amount of human Ang1-7 （Angiotensin 1-7） on the solid phase supporter for sites on the Biotinylated Detection Ab specific to human Ang1-7 （Angiotensin 1-7）. Excess conjugate and unbound sample or standard are washed from the plate，and Avidin conjugated to Horseradish Peroxidase （HRP） are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of human Ang1-7 （Angiotensin 1-7） in the samples is then determined by comparing the OD of the samples to the standard curve.Kit Components：Microtiter Strips，8x12 wellsStandard，2 vialsSample/Standard Diluent Buffer，1x20mlAntibody-Biotin，1x120ulAntibody-Biotin Diluent，1x10mlHRP Conjugate，1x120ulHRP Conjugate Diluent，1x10mlSubstrate Reagent，1x10mlStop Solution，1x10mlWash Buffer （25X），1x30mlStorage and Stability：Unused kit is stable for 6 months after receipt. Store all kit components at 4ºC. Protect HRP Conjugate and Substrate Reagent from light. For maximum recovery，centrifuge the original vials after thawing and prior to removing the cap.
