PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.Intended Use：Suitable for studying enzyme kinetics and HTS applications.Test Principle：The PRMT5 Homogeneous BioAssay Kit is designed to measure PRMT5 activity for screening and profiling applications. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. The PRMT5 Homogeneous BioAssay Kit comes in a convenient AlphaLISA format，with biotinylated histone H4 peptide substrate，primary antibody，methylation assay buffer，and purified PRMT5 for 384 enzyme reactions. The key to the PRMT5 Homogeneous BioAssay Kit is a highly specific antibody that recognizes methylated substrate. With this kit，only three simple steps on a microtiter plate are required for methyltransferase detection. First，a sample containing PRMT5 enzyme is incubated with the biotinylated substrate for two hours. Next，acceptor beads and primary antibody are added，then donor beads，followed by reading the Alpha-counts.Contraindications： Green and blue dyes that absorb light in the AlphaScreen signal emission range （520-620nm），such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide （NaN3） or metal ions （Fe2+，Fe3+，Cu2+，Zn2+ and Ni2+）. The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM，which lacks these components，does not affect AlphaScreen assays.Kit Components：PRMT5/MEP50，2x40ug60uM S-adenosylmethionine，1x250ulPrimary antibody 4-3，1x100ulBiotinylated histone H4 peptide substrate，500 rxns4x PRMT5 assay buffer （add DTT before experiment），3x1ml4x Detection buffer，1x2mlStorage and Stability：Store powder at 4°C liquid at -20°C/-70°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
