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细胞分析试剂盒
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中文名称

PRMT1,Homogeneous BioAssay Kit (HRMT1L2,IR1B4,HCP1)

英文名字
PRMT1,Homogeneous BioAssay Kit(HRMT1L2,IR1B4,HCP1)
供应商
USBiological
产品货号
377003
产品报价
¥1/384Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.Intended Use:Suitable for use in studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.Test Principle:The PRMT1 Homogeneous BioAssay Kit is designed to measure PRMT1 activity for screening and profiling applications. PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3. The PRMT1 Homogeneous BioAssay Kit comes in a convenient AlphaLISA format,with a 384-well plate,biotinylated histone H4 peptide substrate,primary antibody,methylation assay buffer,and purified PRMT1 for 384 enzyme reactions. The key to the PRMT1 Homogeneous BioAssay Kit is a highly specific antibody that recognizes methylated histone substrate. With this kit,only three simple steps on a microtiter plate are required for methyltransferase detection. First,a sample containing PRMT1 enzyme is incubated with the biotinylated substrate for one hour. Next,acceptor beads and primary antibody are added,then donor beads,followed by reading the Alpha- counts.Contraindications:Avoid green and blue dyes that absorb light in the Alphascreen signal emission range (520-620 nm),such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+,Fe3+,Cu2+,Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM,which lacks these components,does not affect Alphascreen assays.Kit Components:PRMT1,1x1ug20uM S-adenosylmethionine,1x500ulPrimary antibody 4,1x400ulBiotinylated histone H4 peptide substrate,1x400ul4x HMT assay Buffer 2A,1x3ml4x Detection Buffer,1x2mlStorage and Stability:Store powder at 4°C liquid at -20°C/-70°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product,centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对PRMT1,Homogeneous BioAssay Kit (HRMT1L2,IR1B4,HCP1)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到PRMT1,Homogeneous BioAssay Kit (HRMT1L2,IR1B4,HCP1)产品后将其置于4°C至-20°C至-70°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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