Intended Use：This BioAssay kit allows for the in vitro quantitative determination of Tylosin concentrations in muscle，liver，honey.Sensitivity：1.5ppbRange：1.5-121.5ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Tylosin antigen. Standards or samples are added to the appropriate microtiter plate wells with Tylosin specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Tylosin and Tylosin in standards or samples with the Tylosin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Tylosin in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96TStandard （96T），6x1mlHRP-conjugate （96T），1x7mlAntibody （96T），1x7mlSubstrate A （96T），1x7mlSubstrate B （96T），1x7mlStop Solution （96T），1x7mlSample Diluent （2X） （96T），1x50mlWash Buffer （20X） （96T），1x40mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
