Sample Type：Honey，tissue （chicken，porcine）Intended Use：This BioAssay allows for the in vitro quantitative determination of Tetracyclines concentrations in honey，tissue （chicken，porcine）.Sensitivity：0.05ppbRange：0.05-4.05ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Tetracyclines antigen. Standards or samples are added to the appropriate microtiter plate wells with Tetracyclines specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Tetracyclines and Tetracyclines in standards or samples with the Tetracyclines special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Tetracyclines in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate （96T），1Concentrated Standard （81ppb），1x1mlHigh Concentration Standard （100ppb），1x1mlHRP-conjugate，1x7mlAntibody，1x7mlSubstrate A，1x7mlSubstrate B，1x7mlStop Solution，1x7mlWash Buffer （20X），1x15mlSample Extraction （10x），2x50mlStandard Redissolving Solution，1x30mlAdhesive Strip，4x stripsInstruction Manual，1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
