Sulfadimidine （SM2） is synthetic antibacterial and plays an important role in controlling and treatment livestock and poultry diseases. Sulfadimidine （SM2） has become a threat for human health and affected the export of animal derived food due to Sulfadimidine （SM2） residues for abuse，not abiding by withdrawal time，entering into the body and ecological system by food chain. Sulfadimidine （SM2） residue limit is 100mg/kg according to No.235 document of the Ministry of Agriculture of the People's Republic of China. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Urine，tissue，milkIntended Use：This BioAssay kit allows for the in vitro quantitative determination of Sulfadimidine （SM2） concentrations in urine，milk，tissue （chicken，porcine，liver）.Sensitivity：1ppbRange：1-81ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Sulfadimidine （SM2） antigen. Standards or samples are added to the appropriate microtiter plate wells with Sulfadimidine （SM2） specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Sulfadimidine （SM2） and Sulfadimidine （SM2） in standards or samples with the Sulfadimidine （SM2） special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Sulfadimidine （SM2） in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （20x） （96T），2x20mlSample DIluent （20x） （48T），1x20mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
