Salbutamol is a short-acting beta2-adrenoreceptor agonist used as antiasthmatic. It can improve the animal of lean meat and reduce fat content by adding micro-Salbutamol into feed，with higher toxicity than Ractopamine，therefore，Salbutamol is banned from using. The method of instrumental analytical is major to detect Salbutamol，but it needs expensive instruments，professional operators and complex pre-treatment; While the method of enzyme linked immunoassay has advantages with simple，rapid，high sensitivity，good specificity and low cost. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Porcine urine 0.1ppb，tissue 5ppb，feed 0.4ppbIntended Use：This BioAssay kit allows for the in vitro quantitative determination of Salbutamol concentrations in urine，feed and tissue （porcine，liver）.Sensitivity：0.1ppbRange：0.1-8.1ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Salbutamol antigen. Standards or samples are added to the appropriate microtiter plate wells with Salbutamol specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Salbutamol and Salbutamol in standards or samples with the Salbutamol special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Salbutamol in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （96T），1x20mlSample DIluent （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
