Ochratoxin A （OTA） is a toxic metabolite produced by Aspergillus flavus and Penicillium genera. Ochratoxin A has a strong liver and kidney toxicity，and has teratogenicity，mutagenicity and carcinogenicity. Ochratoxin A was considered to be the second important and harmful mycotoxin in the family following with Aflatoxin flavus. As the Ochratoxin A producing fungi exist widely in nature，especially higher yield in cereals and cereals products，coffee，cocoa contaminated with Ochratoxin A，it has been potential threat for human health and development of animal husbandry. This kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Feed 5ppb，soybean 5ppbIntended Use：This BioAssay kit allows for the in vitro quantitative determination of Ochratoxin A concentrations in feed，soybean meal.Sensitivity：0.5ppbRange：0.5-40.5ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Ochratoxin A antigen. Standards or samples are added to the appropriate microtiter plate wells with Ochratoxin A specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Ochratoxin A and Ochratoxin A in standards or samples with the Ochratoxin A special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Ochratoxin A in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （20X） （96T），1x20mlSample DIluent （20X） （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
产品特点
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保存建议
建议收到Ochratoxin A （OTA） ELISA BioAssay Kit产品后将其置于4°C至-20°C保存。
