Kanamycin is an aminoglycoside antibiotic and is widely used in treating animal diseases. It harms the 8th cranial nerves，causing damages to the vestibular and cochlear. The main manifestations of renal toxicity are the damages of the proximal convoluted tubule，causing protein urine，hematuresis，renal hypofunction，etc. The residues of Kanamycin in animal derived food affect human health，in China and Occident，Kanamycin has been limited to use for its neurotoxicity and nephrotoxicity. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Milk，milk powder，tissue （chicken，porcine）.Intended Use：This BioAssay kit allows for the in vitro quantitative determination of kanamycin concentrations in milk，milk powder，tissue （chicken，porcine）.Sensitivity：0.5ppbRange：0.5-40.5ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Kanamycin antigen. Standards or samples are added to the appropriate microtiter plate wells with Kanamycin specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Kanamycin and Kanamycin in standards or samples with the Kanamycin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Kanamycin in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （96T），1x20mlSample DIluent （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
