Gentamicin （GEN） belongs to aminoglycoside antibiotics，playing the main role in gram-negative bacteria. It is widely used in veterinary clinical and animal feed additives for its broad antibacterial spectrum，well curative effects and low cost. But it easily causes toxicant side effect during application process，especially in large and non-regulate using，causing Gentamicin residues exceeded in animal derived food，constituting a risk to human health，cranial nerve，hearing and kidney damage. Current methods of detection Gentamicin，such as HPLC or GC-MS，are with sample preparation complex，expensive，difficult and time-consuming. While using the Gentamicin ELISA BioAssay kit is both rapidly and accurately detect Gentamicin residues in samples. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Milk，tissue （porcine，liver，chicken）.Intended Use：This BioAssay kit allows for the in vitro quantitative determination of Gentamicin concentrations in milk，tissue （porcine，liver，chicken）.Sensitivity：0.3ppbRange：0.3-24.3ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Gentamicin antigen. Standards or samples are added to the appropriate microtiter plate wells with Gentamicin specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Gentamicin and Gentamicin in standards or samples with the Gentamicin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Gentamicin in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （96T），1x20mlSample DIluent （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
