Fluoroquinolones drugs belong to chemical synthesis antibacterial and are clinically used to treat urinary tract infections，intestinal tract infections，respiratory tract infections，skin soft tissue infections，peritoneal infections，osteoarticular infections. But in recent years，adverse drug reaction is increasingly prominent as a result of Fluoroquinolones drugs’ wide application，and resistance of human pathogenic bacterial is produced by long-term consumption food containing low Fluoroquinolones drugs residues，thus resulting in bad effect on the treatment of human disease.The method of instrumental analytical is major to detect Fluoroquinolones drugs，but it needs expensive instruments，professional operators and complex pre-treatment; While the method of enzyme linked immunoassay has advantages with simple，rapid，high sensitivity，good specificity and low cost. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Tissue （Chicken，porcine，liver，bovine，mutton，fish，prawn），milk.Intended Use：This BioAssay kit allows for the in vitro quantitative determination of Fluoroquinolones concentrations in milk，tissue （chicken，porcine，liver，fish，shrimp）.Sensitivity：1ppbRange：1-81ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Fluoroquinolon es antigen. Standards or samples are added to the appropriate microtiter plate wells with Fluoroquinolones specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Fluoroquinolones and Fluoroquinolones in standards or samples with the Fluoroquinolones special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Fluoroquinolones in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （96T），1x20mlSample DIluent （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
