Enrofloxacin belongs to chemical synthesis bacteriostatic agent of fluoroquinolones and it has antibacterial effect for both Gram-positive and Gram-negative bacteria. Enrofloxacin is widely distributed in various tissues and its metabolite-ciprofloxacin with strong antibacterial effect. Enrofloxacin has been widely used in animal husbandry and aquaculture. The long-term edible food with low Enrofloxacin residue is harmful to human health and results in occurrence of food-borne disease. The method of instrumental analytical is major to detect Enrofloxacin，but it needs expensive instruments，professional operators and complex pre-treatment，While the method of enzyme linked immunoassay has advantages with simple，rapid，high sensitivity，good specificity and low cost. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Milk，honey，tissue （chicken，porcine，liver，fish，shrimp）.Intended Use：This BioAssay kit allows for the in vitro quantitative determination of Enrofloxacin concentrations in milk，honey，tissue （chicken，porcine，liver，fish，shrimp）.Sensitivity：0.1ppbRange：0.1-8.1ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Enrofloxacin antigen. Standards or samples are added to the appropriate microtiter plate wells with Enrofloxacin specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Enrofloxacin and Enrofloxacin in standards or samples with the Enrofloxacin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Enrofloxacin in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），6x1mlStandard （48T），6x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （96T），1x50mlSample DIluent （48T），1x25mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
